Day 28 31) effects on neuronal responses and pain behaviour were determined. Intra articular injection of 1mg MIA produced stable and robust changes in two measures of pain relevant to clinical OA, and evidence for the presence of central sensitisation was demonstrated. It was also demonstrated that early stage painful responses in this model are not associated with changes in cytokines in the joint.Effects of spinal and systemic administration of nimesulide (3 100IJg in 501JL)on mechanically evoked and post stimulus responses of dorsal horn neurones in MIA treated rats were also measured, as were the effects of spinal cannabinoid receptor antagonism with AM251 (0.1 10IJg in 501JL)and the CB2 receptor antagonist SR144528 (0.001 0.1IJg in 50IJL).
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